BAIT

ARID-1

CELE_Y108G3AL.7, Y108G3AL.7
ARID (AT-rich Interactive Domain-containing protein) homolog
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Caenorhabditis elegans
PREY

SCC-3

CELE_F18E2.3, F18E2.3
scc-3 encodes a cohesin complex subunit homologous to Saccharomyces cerevisiae Irr1p/Scc3p; SCC-3 is required during meiosis for synaptonemal complex formation and sister chromatid cohesion, proper localization of REC-8 to chromosomes, mitotic chromosome segregation, embryonic development, and vulval morphogenesis; in embryos, SCC-3 forms a cohesin complex with SCC-1, SMC-1, SMC-3, and TIM-1, a HEAT/Armadillo repeat-containing protein related to Drosophila TIMELESS; in the germline, SCC-3 associates with chromatin of transistion zone nuclei, assembles along the longitudinal axes of synapsed chromosomes at pachytene, and unlike other cohesins, localizes throughout chromatin at diakinesis; SCC-3 localization requires TIM-1, and SCC-3 and REC-8 are mutually required for chromatin localization.
Caenorhabditis elegans

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SIN-3 acts in distinct complexes to regulate the germline transcriptional program in Caenorhabditis elegans.

Robert VJ, Caron M, Gely L, Adrait A, Pakulska V, Coute Y, Chevalier M, Riedel CG, Bedet C, Palladino F

The transcriptional co-regulator SIN3 influences gene expression through multiple interactions that include histone deacetylases. Haploinsufficiency and mutations in SIN3 are the underlying cause of Witteveen-Kolk syndrome and related intellectual disability and autism syndromes, emphasizing its key role in development. However, little is known about the diversity of its interactions and functions in developmental processes. Here, we show that loss of ... [more]

Development Nov. 01, 2023; 150(21); [Pubmed: 37818613]

Throughput

  • High Throughput

Curated By

  • BioGRID