HNRNPA2B1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PTBP1
Gene Ontology Biological Process
- RNA splicing [TAS]
- gene expression [TAS]
- mRNA processing [TAS]
- mRNA splicing, via spliceosome [TAS]
- negative regulation of RNA splicing [IDA]
- negative regulation of mRNA splicing, via spliceosome [IDA]
- negative regulation of muscle cell differentiation [IDA]
- regulation of alternative mRNA splicing, via spliceosome [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
Cross-linking mass spectrometry discovers, evaluates, and corroborates structures and protein-protein interactions in the human cell.
Significant recent advances in structural biology, particularly in the field of cryoelectron microscopy, have dramatically expanded our ability to create structural models of proteins and protein complexes. However, many proteins remain refractory to these approaches because of their low abundance, low stability, or-in the case of complexes-simply not having yet been analyzed. Here, we demonstrate the power of using cross-linking ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PTBP1 HNRNPA2B1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.846 | BioGRID | 744266 | |
| HNRNPA2B1 PTBP1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 924377 |
Curated By
- BioGRID