An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

PKC?II phosphorylates ACSL4 to amplify lipid peroxidation to induce ferroptosis.

Zhang HL, Hu BX, Li ZL, Du T, Shan JL, Ye ZP, Peng XD, Li X, Huang Y, Zhu XY, Chen YH, Feng GK, Yang D, Deng R, Zhu XF

The accumulation of lipid peroxides is recognized as a determinant of the occurrence of ferroptosis. However, the sensors and amplifying process of lipid peroxidation linked to ferroptosis remain obscure. Here we identify PKC?II as a critical contributor of ferroptosis through independent genome-wide CRISPR-Cas9 and kinase inhibitor library screening. Our results show that PKC?II senses the initial lipid peroxides and amplifies ... [more]

Nat Cell Biol Jan. 01, 2022; 24(1);88-98 [Pubmed: 35027735]

Throughput

Low Throughput

Additional Notes

dimer

Curated By

BioGRID

Interaction Summary

ACSL4

Gene Ontology Biological Process

Gene Ontology Molecular Function

arachidonate-CoA ligase activity [IDA]long-chain fatty acid-CoA ligase activity [IDA]very long-chain fatty acid-CoA ligase activity [IMP]

Gene Ontology Cellular Component