BAIT
ATG8
APG8, AUT7, CVT5, ubiquitin-like protein ATG8, L000004607, L000004604, YBL078C
Component of autophagosomes and Cvt vesicles; regulator of Atg1p, targets it to autophagosomes; binds the Atg1p-Atg13p complex, triggering its vacuolar degradation; unique ubiquitin-like protein whose conjugation target is lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
GO Process (9)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
- CVT pathway [IMP]
- ER to Golgi vesicle-mediated transport [IGI, IMP, IPI]
- autophagic vacuole assembly [IMP]
- cellular protein complex localization [IMP]
- late nucleophagy [IMP]
- membrane fusion [IDA, IMP]
- mitochondrion degradation [IMP]
- piecemeal microautophagy of nucleus [IMP]
- protein targeting to vacuole involved in autophagy [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
TUP1
AAR1, AER2, AMM1, CRT4, CYC9, FLK1, ROX4, SFL2, UMR7, chromatin-silencing transcriptional regulator TUP1, L000002391, YCR084C
General repressor of transcription; forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes
GO Process (10)
GO Function (6)
GO Component (2)
Gene Ontology Biological Process
- histone exchange [IMP]
- negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of mating-type specific transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter by glucose [IMP]
- negative regulation of transcription from RNA polymerase II promoter during mitosis [IMP]
- nucleosome positioning [IDA]
- regulation of fatty acid biosynthetic process by regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of invasive growth in response to glucose limitation [IMP]
- regulation of response to DNA damage stimulus [IMP]
Gene Ontology Molecular Function- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- histone binding [IDA]
- histone deacetylase binding [IDA, IPI]
- mediator complex binding [IDA, IPI]
- phosphatidylinositol-3,5-bisphosphate binding [IDA]
- ubiquitin binding [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- histone binding [IDA]
- histone deacetylase binding [IDA, IPI]
- mediator complex binding [IDA, IPI]
- phosphatidylinositol-3,5-bisphosphate binding [IDA]
- ubiquitin binding [IDA]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An APEX2-based proximity-dependent biotinylation assay with temporal specificity to study protein interactions during autophagy in the yeast Saccharomyces cerevisiae.
Autophagosome biogenesis is a complex process orchestrated by dynamic interactions between Atg (autophagy-related) proteins and characterized by the turnover of specific cargoes, which can differ over time and depending on how autophagy is stimulated. Proteomic analyses are central to uncover protein-protein interaction networks and when combined with proximity-dependent biotinylation or proximity labeling (PL) approaches, they also permit to detect transient ... [more]
Autophagy Jul. 03, 2024; ();1-16 [Pubmed: 38958087]
Throughput
- High Throughput
Additional Notes
- Atg8 interactors upon 1 h of nitrogen starvation
Curated By
- BioGRID