An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

ER-export and ARFRP1/AP-1-dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication.

Pearson GJ, Mears HV, Broncel M, Snijders AP, Bauer DLV, Carlton JG

The ?-coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the global COVID-19 pandemic. Coronaviral Envelope (E) proteins are pentameric viroporins that play essential roles in assembly, release, and pathogenesis. We developed a nondisruptive tagging strategy for SARS-CoV-2 E and find that, at steady state, it localizes to the Golgi and to lysosomes. We identify sequences ... [more]

Sci Adv Apr. 05, 2024; 10(14);eadl5012 [Pubmed: 38569033]

Throughput

High Throughput

Additional Notes

TurboID

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
E ABCC1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Samavarchi-Tehrani P (2020)	High	16.88	BioGRID	2790293
E ABCC1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	St-Germain JR (2020)	High	25	BioGRID	2805565

Curated By

BioGRID

Interaction Summary

E

ABCC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [IDA]transporter activity [TAS]

Gene Ontology Cellular Component

Proximity Label-MS