BAIT

NSP12

ORF1ab, ORF1ab-nsp12, SARS-CoV2 nsp12, SARS-CoV-2 nsp12, RNA-directed RNA polymerase, R1AB_SARS2, PRO_0000449629, GU280_gp01_nsp12
RNA-directed RNA polymerase; Non-structural protein 12
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

CCT8

C21orf112, Cctq, D21S246, PRED71
chaperonin containing TCP1, subunit 8 (theta)
Cell Cycle - Mitotic Spindle Assembly Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SARS-CoV-2 NSP12 associates with TRiC and the P323L substitution acts as a host adaption.

Alruwaili M, Armstrong S, Prince T, Erdmann M, Matthews DA, Luu L, Davidson A, Aljabr W, Hiscox JA

SARS-CoV-2 has caused a worldwide health and economic crisis. During the course of the pandemic, genetic changes occurred in the virus, which have resulted in new properties of the virus-particularly around gains in transmission and the ability to partially evade either natural or vaccine-acquired immunity. Some of these viruses have been labeled Variants of Concern (VoCs). At the root of ... [more]

J Virol Nov. 30, 2023; 97(11);e0042423 [Pubmed: 37929963]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture MS was carried out to identify high confidence protein interactors for NSP12 L323 (B.1) with a BFDR less than or equal to 0.05, MiST score greater than or equal to 0.6, and two or more unique peptides
  • Affinity capture MS was carried out to identify high confidence protein interactors for NSP12 P323 (WT) with a BFDR less than or equal to 0.05, MiST score greater than or equal to 0.6, and two or more unique peptides

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP12 CCT8
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
3746617
NSP12 CCT8
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2798881

Curated By

  • BioGRID