N
TARBP2
Gene Ontology Biological Process
- gene expression [TAS]
- miRNA loading onto RISC involved in gene silencing by miRNA [IDA]
- negative regulation of defense response to virus by host [IDA]
- negative regulation of protein kinase activity [TAS]
- positive regulation of viral genome replication [IDA]
- pre-miRNA processing [IDA]
- production of siRNA involved in RNA interference [IDA]
- regulation of transcription from RNA polymerase II promoter [TAS]
- regulation of transcription, DNA-templated [TAS]
- regulation of viral transcription [IDA]
- targeting of mRNA for destruction involved in RNA interference [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Protein-RNA
An interaction is detected between and protein and an RNA in vitro.
Publication
High-sensitivity profiling of SARS-CoV-2 noncoding region-host protein interactome reveals the potential regulatory role of negative-sense viral RNA.
A deep understanding of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-host interactions is crucial to developing effective therapeutics and addressing the threat of emerging coronaviruses. The role of noncoding regions of viral RNA (ncrRNAs) has yet to be systematically scrutinized. We developed a method using MS2 affinity purification coupled with liquid chromatography-mass spectrometry and designed a diverse set of bait ... [more]
Throughput
- High Throughput
Additional Notes
- Protein RNA - MS was carried out to identify high confidence protein interactors for the SARS-CoV-2 ncrRNA NRC6 (5' UTR of N)
- Protein RNA - MS was carried out to identify high confidence protein interactors for the SARS-CoV-2 ncrRNA NRC7 (negative sense of NRC6 [5' UTR of N])
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| N TARBP2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3459033 | |
| N TARBP2 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 2798762 | |
| TARBP2 N | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 3501233 | |
| N TARBP2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 3501013 |
Curated By
- BioGRID