An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

PAICS ubiquitination recruits UBAP2 to trigger phase separation for purinosome assembly.

Chou MC, Wang YH, Chen FY, Kung CY, Wu KP, Kuo JC, Chan SJ, Cheng ML, Lin CY, Chou YC, Ho MC, Firestine S, Huang JR, Chen RH

Purinosomes serve as metabolons to enhance de novo purine synthesis (DNPS) efficiency through compartmentalizing DNPS enzymes during stressed conditions. However, the mechanism underpinning purinosome assembly and its pathophysiological functions remains elusive. Here, we show that K6-polyubiquitination of the DNPS enzyme phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthetase (PAICS) by cullin-5/ankyrin repeat and SOCS box containing 11 (Cul5/ASB11)-based ubiquitin ligase plays a driving ... [more]

Mol Cell Nov. 16, 2023; 83(22);4123-4140.e12 [Pubmed: 37848033]

Throughput

High Throughput

Additional Notes

Proximity label MS was carried out to identify high confidence protein interactors of WT K74-Ubiquitinated PAICS (in comparison to K74R mutant)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FXR2 PAICS	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Rual JF (2005)	High	-	BioGRID	-
FXR2 PAICS	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Sakai Y (2011)	High	-	BioGRID	-
FXR2 PAICS	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Venkatesan K (2009)	High	-	BioGRID	838760

Curated By

BioGRID

Interaction Summary

PAICS

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]phosphoribosylaminoimidazole carboxylase activity [TAS]phosphoribosylaminoimidazolesuccinocarboxamide synthase activity [TAS]protein binding [IPI]

Gene Ontology Cellular Component

FXR2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [TAS]identical protein binding [IPI]mRNA binding [IBA]poly(A) RNA binding [IDA]protein binding [IPI]