Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

An empirical framework for binary interactome mapping.

Venkatesan K, Rual JF, Vazquez A, Stelzl U, Lemmens I, Hirozane-Kishikawa T, Hao T, Zenkner M, Xin X, Goh KI, Yildirim MA, Simonis N, Heinzmann K, Gebreab F, Sahalie JM, Cevik S, Simon C, de Smet AS, Dann E, Smolyar A, Vinayagam A, Yu H, Szeto D, Borick H, Dricot A, Klitgord N, Murray RR, Lin C, Lalowski M, Timm J, Rau K, Boone C, Braun P, Cusick ME, Roth FP, Hill DE, Tavernier J, Wanker EE, Barabasi AL, Vidal M

Several attempts have been made to systematically map protein-protein interaction, or 'interactome', networks. However, it remains difficult to assess the quality and coverage of existing data sets. Here we describe a framework that uses an empirically-based approach to rigorously dissect quality parameters of currently available human interactome maps. Our results indicate that high-throughput yeast two-hybrid (HT-Y2H) interactions for human proteins ... [more]

Nat. Methods Jan. 01, 2009; 6(1);83-90 [Pubmed: 19060904]

Throughput

High Throughput

Additional Notes

Human proteins expressed in S. cerevisiae

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PAICS FXR2	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Chou MC (2023)	High	-	BioGRID	3758017
FXR2 PAICS	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Rual JF (2005)	High	-	BioGRID	-
FXR2 PAICS	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Sakai Y (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FXR2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [TAS]identical protein binding [IPI]mRNA binding [IBA]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

PAICS

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]phosphoribosylaminoimidazole carboxylase activity [TAS]phosphoribosylaminoimidazolesuccinocarboxamide synthase activity [TAS]protein binding [IPI]