BAIT

C18ORF25

ARKL1, RNF111L1
chromosome 18 open reading frame 25
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

PML

MYL, PP8675, RNF71, TRIM19
promyelocytic leukemia
GO Process (39)
GO Function (8)
GO Component (9)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A CK2 and SUMO-dependent, PML NB-involved regulatory mechanism controlling BLM ubiquitination and G-quadruplex resolution.

Liu S, Atkinson E, Paulucci-Holthauzen A, Wang B

The Boom syndrome helicase (BLM) unwinds a variety of DNA structures such as Guanine (G)-quadruplex. Here we reveal a role of RNF111/Arkadia and its paralog ARKL1, as well as Promyelocytic Leukemia Nuclear Bodies (PML NBs), in the regulation of ubiquitination and control of BLM protein levels. RNF111 exhibits a non-canonical SUMO targeted E3 ligase (STUBL) activity targeting BLM ubiquitination in ... [more]

Nat Commun Sep. 30, 2023; 14(1);6111 [Pubmed: 37777511]

Throughput

  • Low Throughput

Curated By

  • BioGRID