Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Phase separation of initiation hubs on cargo is a trigger switch for selective autophagy.

Licheva M, Pflaum J, Babic R, Mancilla H, Elsaesser J, Boyle E, Hollenstein DM, Jimenez-Niebla J, Pleyer J, Heinrich M, Wieland FG, Brenneisen J, Eickhorst C, Brenner J, Jiang S, Hartl M, Welsch S, Hunte C, Timmer J, Wilfling F, Kraft C

Autophagy is a key cellular quality control mechanism. Nutrient stress triggers bulk autophagy, which nonselectively degrades cytoplasmic material upon formation and liquid-liquid phase separation of the autophagy-related gene 1 (Atg1) complex. In contrast, selective autophagy eliminates protein aggregates, damaged organelles and other cargoes that are targeted by an autophagy receptor. Phase separation of cargo has been observed, but its regulation ... [more]

Nat Cell Biol Feb. 01, 2025; 27(2);283-297 [Pubmed: 39774270]

Throughput

  • High Throughput

Curated By

  • BioGRID