BAIT

CDC48

AAA family ATPase CDC48, L000000280, YDL126C
AAA ATPase; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing, in macroautophagy, PMN, RAD, ribophagy, homotypic ER membrane fusion, disassembly of Met30p from SCF complex; functional ortholog of human p97/VCP
GO Process (18)
GO Function (3)
GO Component (11)
Saccharomyces cerevisiae (S288c)
PREY

CDC55

TMR4, protein phosphatase 2A regulatory subunit CDC55, L000000282, S000029602, L000003191, YGL190C
Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion; homolog of mammalian B55
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Cdc48 plays a crucial role in redox homeostasis through dynamic reshaping of its interactome during early stationary phase.

Radzinski M, Oppenheim T, Yogev O, Levy A, Naomi MB, Kacen A, Merbl Y, Ravid T, Reichmann D

Most microbial cells on earth predominantly exist in non-proliferating, dormant conditions, such as the stationary state. The stationary phase is a crucial stage during the cellular lifespan, which requires homeostatic rewiring for long-term viability and rapid responses to environmental changes. Here, we show that entry to the stationary phase in yeast is accompanied by increased cytosolic and mitochondrial oxidation, imposing ... [more]

Redox Biol Jul. 01, 2025; 84();103651 [Pubmed: 40359616]

Throughput

  • High Throughput

Additional Notes

  • Cdc48-interacting proteins at 24 hours and 48 hours growth

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CDC55 CDC48
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2589BioGRID
2043699

Curated By

  • BioGRID