An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The solute carrier superfamily interactome.

Frommelt F, Ladurner R, Goldmann U, Wolf G, Ingles-Prieto A, Lineiro-Retes E, Gelova Z, Hopp AK, Christodoulaki E, Teoh ST, Leippe P, Santini BL, Rebsamen M, Lindinger S, Serrano I, Onstein S, Klimek C, Barbosa B, Pantielieieva A, Dvorak V, Hannich TJ, Schoenbett J, Sansig G, Mocking TAM, Ooms JF, IJzerman AP, Heitman LH, Sykacek P, Reinhardt J, Mueller AC, Wiedmer T, Superti-Furga G

Solute carrier (SLC) transporters form a protein superfamily that enables transmembrane transport of diverse substrates including nutrients, ions and drugs. There are about 450 different SLCs, residing in a variety of subcellular membranes. Loss-of-function of an unusually high proportion of SLC transporters is genetically associated with a plethora of human diseases, making SLCs a rapidly emerging but challenging drug target ... [more]

Mol Syst Biol Jun. 01, 2025; 21(6);632-675 [Pubmed: 40355756]

Quantitative Score

0.402505623 [Confidence Score]

Throughput

High Throughput

Additional Notes

CompPASS and SAINTexpress scores were integrated with quantitative data using a multi-modal RBF classifier (Frommelt et al., Mol. Syst. Biol., 2025, PMID: 40355756).
HEK 293 Jump-In cells
PPI probability score = 0.402505622573339

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
STK39 SLC12A2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Piechotta K (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SLC12A2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ammonium transmembrane transporter activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

STK39