Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

MST1 interactomes profiling across cell death in esophageal squamous cell carcinoma.

Zhang L, Gao M, Wu Y, Liu H, Zhuang X, Zhou Y, Song Q, Bi S, Zhang W, Cui Y

Resistance to apoptosis in esophageal squamous cell carcinoma (ESCC) constitutes a significant impediment to treatment efficacy. Exploring alternative cell death pathways and their regulatory factors beyond apoptosis is crucial for overcoming drug resistance and enhancing therapeutic outcomes in ESCC.Mammalian Ste 20-like kinase 1 (MST1) is implicated in regulating various cell deaths, including apoptosis, autophagy, and pyroptosis. Employing enhanced ascorbate peroxidase ... [more]

Med Rev (2021) Dec. 01, 2024; 4(6);531-543 [Pubmed: 39664081]

Throughput

  • High Throughput

Additional Notes

  • Interaction observed under cisplatin (DPP) (CHEBI:27899) treatment, apoptosis interactome
  • Interaction observed under rapamycin (CHEBI:9168) treatment, autophagy interactome
  • Interaction observed under trichostatin A (TSA) (CHEBI:46024) treatment, pyroptosis interactome
  • Interaction observed with no chemical treatment, steady-state interactome

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
STK4 KIF2A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.84BioGRID
-
STK4 KIF2A
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3827356

Curated By

  • BioGRID