BAIT

SCS2

phosphatidylinositol-binding protein SCS2, L000002629, YER120W
Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

STT4

1-phosphatidylinositol 4-kinase STT4, L000002137, YLR305C
Phosphatidylinositol-4-kinase; functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Maintenance of proper phosphatidylinositol-4-phosphate level by Stt4 and Sac1 contributes to vesicular transport to and from the plasma membrane.

Sano T, Nagano M, Shimamura H, Yamamoto W, Tamada T, Toshima JY, Toshima J

Growing evidence suggests that counter-transport of phosphatidylinositol-4-phosphate (PtdIns(4)P) and phosphatidylserine (PS) at endoplasmic reticulum (ER)-plasma membrane (PM) contact sites is required for intracellular vesicle transport. PtdIns(4)P is metabolized by Stt4 PI 4-kinase residing at the PM and by Sac1 PtdIns(4)P phosphatase at the ER, and ER-PM contact sites are believed to be important for its efficient turnover. Recently, Stt4 has ... [more]

J Biol Chem Aug. 01, 2025; 301(8);110410 [Pubmed: 40550329]

Throughput

  • Low Throughput

Ontology Terms

  • chemical compound accumulation (APO:0000095)

Additional Notes

  • Genetic complex
  • stt4 mutation suppresses PtdIns(4)P levels at the plasma membrane in Ist2/ Scs2/ Scs22/ Tcb1/ Tcb2/ Tcb3 mutants

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SCS2 STT4
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
SCS2 STT4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1666BioGRID
2040660
STT4 SCS2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.5167BioGRID
2002411
STT4 SCS2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1789BioGRID
2427064
STT4 SCS2
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

High-BioGRID
204997

Curated By

  • BioGRID