Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

E3 ligase AREL1 controls perinuclear localization of lysosomes and supports Purkinje cell survival.

Jiang L, Tang J, Zhang YF, Zou WX, Deng G, Tian N, Zhao X, Han L, Liu K, Song BL, Luo J

Localization of lysosomes influences their properties, e.g., perinuclear lysosomes are more acidic but less mobile compared with the peripheral ones. Furthermore, the endoplasmic reticulum (ER) can actively regulate the dynamics and functions of lysosomes via membrane contact sites. In this study, we find that ER-resident apoptosis-resistant E3 ubiquitin protein ligase 1 (AREL1) establishes membrane contacts with lysosomes by directly interacting ... [more]

EMBO J Dec. 02, 2025; (); [Pubmed: 41331534]

Throughput

  • Low Throughput

Additional Notes

  • Proximity Ligation Assay to measure ER-endolysosomal membrane contact

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CANX LAMP1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3349574

Curated By

  • BioGRID