TPX2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TOLLIP
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
Centrosome clustering in cancer cells requires microtubule assembly through a RanGTP-dependent TPX2-KIFC1 interaction.
Aneuploidy, chromosomal instability (CIN), and centrosome amplification are hallmarks of aggressive solid tumors. Cancer cells with supernumerary centrosomes ensure bipolar spindle formation by efficiently clustering them at the spindle poles. TPX2 (targeting protein for Xenopus kinesin-like protein 2), a nuclear and microtubule-associated protein, and its partner, the Aurora-A kinase (AURKA), are key mitotic players frequently co-overexpressed in human cancers. TPX2 ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TOLLIP TPX2 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3307892 |
Curated By
- BioGRID