Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

Quantitative interactome analysis reveals a chemoresistant edgotype.

Chavez JD, Schweppe DK, Eng JK, Zheng C, Taipale A, Zhang Y, Takara K, Bruce JE

Chemoresistance is a common mode of therapy failure for many cancers. Tumours develop resistance to chemotherapeutics through a variety of mechanisms, with proteins serving pivotal roles. Changes in protein conformations and interactions affect the cellular response to environmental conditions contributing to the development of new phenotypes. The ability to understand how protein interaction networks adapt to yield new function or ... [more]

Nat Commun Aug. 03, 2015; 6();7928 [Pubmed: 26235782]

Throughput

  • High Throughput

Ontology Terms

  • uterine cervix (BTO:0001421)
  • hela cell (BTO:0000567) [cervical adenocarcinoma cell (BTO:0003165)]
  • epithelial cell of cervix (CL:0002535)

Additional Notes

  • False discovery rate (FDR) =< 1%
  • HELA cells
  • Method used identifies inter-protein and homooligomer interactions.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ANXA1 ANXA1
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
659097
ANXA1 ANXA1
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

High-BioGRID
-

Curated By

  • BioGRID