BAIT

RTG3

L000003152, YBL103C
bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

YIM1

L000002671, YMR152W
Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; may have a role in lipid metabolism, based on localization to lipid droplets; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
GO Process (1)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Global mitochondrial connectivity map reveals the landscape of yeast functional assemblies and conserved protein communities.

Jessulat M, Phanse S, Aoki H, Broderick K, Zhang Q, Castro IG, Novales NA, Hossain SA, Saccon T, Moutaoufik MT, Joseph TP, Amin S, Hoell L, Minic Z, Bykov Y, Preminger N, Crespo SG, Snider J, Golshani A, Stagljar I, Rodriguez-Medina JR, Clarke CF, Schuldiner M, Babu M

Mitochondria are essential organelles whose functions depend on coordinated multiprotein complexes, yet their composition and organization remain incomplete. Here, we present a large-scale map of mitochondrial protein complexes by integrating affinity purification of 740 endogenously GFP-tagged mitochondrial proteins with biochemical co-fractionation of mitochondrial extracts from yeast (Saccharomyces cerevisiae) grown under respiratory conditions. Mass spectrometry identifies 13,716 high-confidence protein associations and ... [more]

Nat Commun May. 05, 2026; (); [Pubmed: 42086547]

Quantitative Score

  • 8.19 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • High throughput score cut-off value/description The PPIs are derived from two methods AP-MS and CF-MS. The LLS PPI score is derived from the LLS AP-MS and LLS CF-MS scores. The cutoff for AP-MS is 5.34 and CF-MS is 3.12. If the PPI is detected by both methods and meets the threshold in one of the methods the Average LLS score is taken as the final LLS score for the PPI.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RTG3 YIM1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-2.52BioGRID
2358022

Curated By

  • BioGRID