U2AF2
Gene Ontology Biological Process
- RNA splicing [TAS]
- gene expression [TAS]
- mRNA 3'-end processing [TAS]
- mRNA export from nucleus [TAS]
- mRNA processing [TAS]
- mRNA splicing, via spliceosome [IC, TAS]
- negative regulation of mRNA splicing, via spliceosome [IDA]
- positive regulation of RNA splicing [IDA]
- termination of RNA polymerase II transcription [TAS]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SUGP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Construction of a proximity labeling vector to identify protein-protein interactions in human stem cells.
Identification of protein-protein interactions is essential for understanding protein functions in biological processes. While immunoprecipitation has traditionally been used to isolate proteins and their partners, it faces limitations in capturing transient interactions. Proximity labeling, particularly with the biotin ligase TurboID, addresses this challenge by enabling rapid and efficient identification of interacting proteins in vivo. Human induced pluripotent stem cells are ... [more]
Throughput
- High Throughput
Additional Notes
- A cutoff of p-value lesser of equal to 0.05 and a Log2deltaLFQ difference greater or equal to 2 was used to define true interactions
- mesoderm stage
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| U2AF2 SUGP1 | Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag. | Low | - | BioGRID | - | |
| SUGP1 U2AF2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| U2AF2 SUGP1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| SUGP1 U2AF2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID