BAIT

DISC1

C1orf136, SCZD9, RP4-730B13.1
disrupted in schizophrenia 1
GO Process (4)
GO Function (1)
GO Component (2)
Homo sapiens
PREY

PAFAH1B1

LIS1, LIS2, MDCR, MDS, PAFAH
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

A peptide mimetic therapeutic strategy targeting dysfunction of the scaffold protein DISC-1 in psychiatric disorders.

Cukkemane A, Dingley AJ, Mohrlueder J, Santiago-Schuebel B, Weiergraeber OH, Willbold D

Disrupted in schizophrenia 1 (DISC1) is a scaffold protein that regulates several physiological processes ranging from cellular division to neurodevelopment, and its dysfunction contributes to various neurological disorders including schizophrenia, bipolar and mood disorders, and autism. Thus, deciphering its native functions and pathophysiological roles is crucial. In this report, three disease-associated mutants of the C-region of DISC1, i.e., S713E, S704C, ... [more]

Eur J Pharm Sci Aug. 01, 2025; 211();107148 [Pubmed: 40449672]

Throughput

  • Low Throughput

Additional Notes

  • DISC1 C-region
  • bio-layer interferometry

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DISC1 PAFAH1B1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.5589BioGRID
3218773
DISC1 PAFAH1B1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2606554
DISC1 PAFAH1B1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
2606557
DISC1 PAFAH1B1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID