BAIT

RANGAP1

Fug1, RANGAP, SD, RP4-756G23.7
Ran GTPase activating protein 1
GO Process (3)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

BAG1

BAG-1, HAP, RAP46, RP11-326F20.2
BCL2-associated athanogene
Alzheimer's Disease Project
GO Process (4)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

Enhanced protein-protein interaction network construction promoted by in vivo cross-linking with acid-cleavable click-chemistry enrichment.

Zhao L, Zhong B, An Y, Zhang W, Gao H, Zhang X, Liang Z, Zhang Y, Zhao Q, Zhang L

Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enables global profiling of protein interactome with direct interaction interfaces in complex biological systems. The alkyne-tagged enrichable cross-linkers are preferred to improve the coverage of low-abundance cross-linked peptides, combined with click chemistry for biotin conjugation to allow the cross-linked peptide enrichment. However, a systematic evaluation on the ... [more]

Front Chem Dec. 09, 2022; 10();994572 [Pubmed: 36479438]

Throughput

  • High Throughput

Ontology Terms

  • hek-293t cell (BTO:0002181) [embryonic kidney cell line (BTO:0002733)]
  • kidney epithelial cell (CL:0002518)
  • kidney (BTO:0000671)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BAG1 RANGAP1
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High-BioGRID
924569
RANGAP1 BAG1
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
3721084

Curated By

  • BioGRID