An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.

Jeronimo C, Forget D, Bouchard A, Li Q, Chua G, Poitras C, Therien C, Bergeron D, Bourassa S, Greenblatt J, Chabot B, Poirier GG, Hughes TR, Blanchette M, Price DH, Coulombe B

We have performed a survey of soluble human protein complexes containing components of the transcription and RNA processing machineries using protein affinity purification coupled to mass spectrometry. Thirty-two tagged polypeptides yielded a network of 805 high-confidence interactions. Remarkably, the network is significantly enriched in proteins that regulate the formation of protein complexes, including a number of previously uncharacterized proteins for ... [more]

Mol. Cell Jul. 20, 2007; 27(2);262-74 [Pubmed: 17643375]

Quantitative Score

0.995909 [Confidence Score]

Throughput

High Throughput

Ontology Terms

hek-293 cell (BTO:0000007)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HNRNPA1 ZFR	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Close P (2012)	High	-	BioGRID	669415
HNRNPA1 ZFR	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2015)	High	0.9755	BioGRID	1184513
HNRNPA1 ZFR	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	0.8955	BioGRID	2259363
HNRNPA1 ZFR	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9434	BioGRID	3069138
HNRNPA1 ZFR	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	9.76	BioGRID	2990439

Curated By

BioGRID

Interaction Summary

HNRNPA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]single-stranded DNA binding [IDA]single-stranded RNA binding [IC]

Gene Ontology Cellular Component

ZFR

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]