ERCC3
Gene Ontology Biological Process
- 7-methylguanosine mRNA capping [TAS]
- DNA repair [IMP, TAS]
- DNA topological change [IMP]
- apoptotic process [IMP]
- gene expression [TAS]
- hair cell differentiation [IMP]
- nucleotide-excision repair [IMP, TAS]
- nucleotide-excision repair, DNA damage removal [TAS]
- nucleotide-excision repair, DNA duplex unwinding [IMP]
- nucleotide-excision repair, DNA incision [IMP]
- positive regulation of apoptotic process [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of viral transcription [TAS]
- protein localization [IMP]
- regulation of mitotic cell cycle phase transition [IMP]
- response to UV [IMP]
- response to oxidative stress [IMP]
- termination of RNA polymerase I transcription [TAS]
- transcription elongation from RNA polymerase I promoter [TAS]
- transcription elongation from RNA polymerase II promoter [TAS]
- transcription from RNA polymerase I promoter [TAS]
- transcription from RNA polymerase II promoter [IDA, IMP, TAS]
- transcription initiation from RNA polymerase I promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription-coupled nucleotide-excision repair [IDA, TAS]
- viral process [TAS]
Gene Ontology Molecular Function- 3'-5' DNA helicase activity [IDA, IMP]
- ATPase activity [IDA]
- DNA binding [TAS]
- DNA-dependent ATPase activity [IDA, IMP]
- RNA polymerase II carboxy-terminal domain kinase activity [IDA]
- damaged DNA binding [NAS]
- protein C-terminus binding [IPI]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [IDA]
- transcription factor binding [IDA]
- 3'-5' DNA helicase activity [IDA, IMP]
- ATPase activity [IDA]
- DNA binding [TAS]
- DNA-dependent ATPase activity [IDA, IMP]
- RNA polymerase II carboxy-terminal domain kinase activity [IDA]
- damaged DNA binding [NAS]
- protein C-terminus binding [IPI]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [IDA]
- transcription factor binding [IDA]
Gene Ontology Cellular Component
- holo TFIIH complex [IDA, TAS]
- nucleoplasm [IDA, TAS]
- nucleus [TAS]
BCR
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.
We have performed a survey of soluble human protein complexes containing components of the transcription and RNA processing machineries using protein affinity purification coupled to mass spectrometry. Thirty-two tagged polypeptides yielded a network of 805 high-confidence interactions. Remarkably, the network is significantly enriched in proteins that regulate the formation of protein complexes, including a number of previously uncharacterized proteins for ... [more]
Quantitative Score
- 0.789801 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ERCC3 BCR | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2221848 | |
| ERCC3 BCR | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3059362 | |
| ERCC3 BCR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| BCR ERCC3 | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | 244049 | |
| ERCC3 BCR | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| BCR ERCC3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID