BAIT

TAF9

TAF17, TATA-binding protein-associated factor TAF9, TafII17, L000003459, YMR236W
Subunit (17 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
Saccharomyces cerevisiae (S288c)
PREY

CTK3

L000003012, YML112W
Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

TFIID and Spt-Ada-Gcn5-acetyltransferase functions probed by genome-wide synthetic genetic array analysis using a Saccharomyces cerevisiae taf9-ts allele.

Milgrom E, West RW, Gao C, Shen WC

TAF9 is a TATA-binding protein associated factor (TAF) conserved from yeast to humans and shared by two transcription coactivator complexes, TFIID and SAGA. The essentiality of the TAFs has made it difficult to ascertain their roles in TFIID and SAGA function. Here we performed a genomic synthetic genetic array analysis using a temperature-sensitive allele of TAF9 as a query. Results ... [more]

Genetics Nov. 01, 2005; 171(3);959-73 [Pubmed: 16118188]

Throughput

  • High Throughput|Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • Genetic interactions were scored as strong when growth defects were observed at both 30 and 34 degrees Celsius, and scored as conditional (heat sensitivity) when growth defects were observed at 34 degrees, but not at 30 degrees.
  • High Throughput: SGA analysis was performed with a temperature-sensitive taf9-ts2 allele as the query.
  • Low Throughput: Genetic interactions identified using an SGA screen were subsequently confirmed by tetrad analysis.

Curated By

  • BioGRID