BAIT

SRB5

MED18, L000002052, YGR104C

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance

GO Process (4)

GO Function (3)

GO Component (1)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IDA]

positive regulation of transcription from RNA polymerase II promoter [IDA]

termination of RNA polymerase II transcription [IMP]

transcriptional open complex formation at RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
core RNA polymerase II binding transcription factor activity [IDA, IGI]

Gene Ontology Cellular Component

core mediator complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SIN4

BEL2, GAL22, MED16, RYE1, SDI3, SSF5, SSN4, SSX3, TSF3, L000001888, S000029516, L000002099, YNL236W

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription

GO Process (3)

GO Function (1)

GO Component (2)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IMP]

negative regulation of transcription from RNA polymerase II promoter [IMP]

positive regulation of transcription from RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IDA]

Gene Ontology Cellular Component

core mediator complex [IDA]

mediator complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Preparation and topology of the Mediator middle module.

Koschubs T, Lorenzen K, Baumli S, Sandstroem S, Heck AJ, Cramer P

Mediator is the central coactivator complex required for regulated transcription by RNA polymerase (Pol) II. Mediator consists of 25 subunits arranged in the head, middle, tail and kinase modules. Structural and functional studies of Mediator are limited by the availability of protocols for the preparation of recombinant modules. Here, we describe protocols for obtaining pure endogenous and recombinant complete Mediator ... [more]

Nucleic Acids Res. Jun. 01, 2010; 38(10);3186-95 [Pubmed: 20123732]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SRB5 SIN4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
SRB5 SIN4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Koschubs T (2009)	Low	-	BioGRID	-
SRB5 SIN4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2004)	High	-	BioGRID	-
SRB5 SIN4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
SRB5 SIN4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Liu Y (2001)	Low	-	BioGRID	-
SRB5 SIN4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	3	BioGRID	3618749
SRB5 SIN4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Liu Z (2012)	Low	-	BioGRID	-
SRB5 SIN4	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Baidoobonso SM (2007)	Low	-	BioGRID	-
SRB5 SIN4	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Liu Y (2001)	Low	-	BioGRID	-
SIN4 SRB5	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Chang M (1999)	Low	-	BioGRID	158815

Curated By

BioGRID

Interaction Summary

SRB5

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]core RNA polymerase II binding transcription factor activity [IDA, IGI]

Gene Ontology Cellular Component

SIN4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-Western

Publication

Preparation and topology of the Mediator middle module.

Throughput

Related interactions

Curated By

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
core RNA polymerase II binding transcription factor activity [IDA, IGI]