BAIT

SRB5

MED18, L000002052, YGR104C

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance

GO Process (4)

GO Function (3)

GO Component (1)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IDA]

positive regulation of transcription from RNA polymerase II promoter [IDA]

termination of RNA polymerase II transcription [IMP]

transcriptional open complex formation at RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
core RNA polymerase II binding transcription factor activity [IDA, IGI]

Gene Ontology Cellular Component

core mediator complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

CSE2

MED9, L000000426, YNR010W

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity; relocalizes to the cytosol in response to hypoxia

GO Process (2)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

negative regulation of transcription from RNA polymerase II promoter [IMP]

positive regulation of transcription from RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IDA]
structural molecule activity [IMP]

Gene Ontology Cellular Component

core mediator complex [IDA]

cytosol [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Preparation and topology of the Mediator middle module.

Koschubs T, Lorenzen K, Baumli S, Sandstroem S, Heck AJ, Cramer P

Mediator is the central coactivator complex required for regulated transcription by RNA polymerase (Pol) II. Mediator consists of 25 subunits arranged in the head, middle, tail and kinase modules. Structural and functional studies of Mediator are limited by the availability of protocols for the preparation of recombinant modules. Here, we describe protocols for obtaining pure endogenous and recombinant complete Mediator ... [more]

Nucleic Acids Res. Jun. 01, 2010; 38(10);3186-95 [Pubmed: 20123732]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SRB5 CSE2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Koschubs T (2009)	Low	-	BioGRID	-
SRB5 CSE2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
SRB5 CSE2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Liu Y (2001)	Low	-	BioGRID	-
SRB5 CSE2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3618746
CSE2 SRB5	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Han SJ (2001)	Low	-	BioGRID	-
SRB5 CSE2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Liu Z (2012)	Low	-	BioGRID	-
SRB5 CSE2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zhu X (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SRB5

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]core RNA polymerase II binding transcription factor activity [IDA, IGI]

Gene Ontology Cellular Component

CSE2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IDA]structural molecule activity [IMP]

Gene Ontology Cellular Component

Affinity Capture-Western

Publication

Preparation and topology of the Mediator middle module.

Throughput

Related interactions

Curated By

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
core RNA polymerase II binding transcription factor activity [IDA, IGI]

RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IDA]
structural molecule activity [IMP]