BAIT

PFY1

CLS5, PRF1, profilin, L000001407, YOR122C
Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress; highly conserved protein; human PFN1 (profilin 1) complements temperature sensitive pfy1 mutants, PFN1 mutations are a rare cause of ALS
Saccharomyces cerevisiae (S288c)
PREY

SMY1

L000001940, YKL079W
Kinesin-like myosin passenger-protein; interacts with Myo2p; controls actin cable structure and dynamics; proposed to be involved in exocytosis
GO Process (1)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Suppression of the profilin-deficient phenotype by the RHO2 signaling pathway in Saccharomyces cerevisiae.

Marcoux N, Cloutier S, Zakrzewska E, Charest PM, Bourbonnais Y, Pallotta D

Profilin plays an important role in actin organization in all eukaryotic cells through mechanisms that are still poorly understood. We had previously shown that Mid2p, a transmembrane protein and a potential cell wall sensor, is an effective multicopy suppressor of the profilin-deficient phenotype in Saccharomyces cerevisiae. To better understand the role of Mid2p in the organization of the actin cytoskeleton, ... [more]

Genetics Oct. 01, 2000; 156(2);579-92 [Pubmed: 11014808]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: budding pattern (APO:0000200)
  • phenotype: heat sensitivity (APO:0000147)
  • phenotype: actin cytoskeleton morphology (APO:0000120)
  • phenotype: resistance to chemicals (APO:0000087)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SMY1 PFY1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2033BioGRID
2053862

Curated By

  • BioGRID