BAIT

SRB5

MED18, L000002052, YGR104C

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance

GO Process (4)

GO Function (3)

GO Component (1)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IDA]

positive regulation of transcription from RNA polymerase II promoter [IDA]

termination of RNA polymerase II transcription [IMP]

transcriptional open complex formation at RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
core RNA polymerase II binding transcription factor activity [IDA, IGI]

Gene Ontology Cellular Component

core mediator complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RPB4

CTF15, DNA-directed RNA polymerase II subunit RPB4, B32, L000001678, YJL140W

RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation

GO Process (6)

GO Function (4)

GO Component (4)

Gene Ontology Biological Process

mRNA export from nucleus in response to heat stress [IMP]

nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IMP]

positive regulation of translational initiation [IMP]

recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [IMP]

transcription from RNA polymerase II promoter [IMP]

transcription initiation from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

RNA-directed RNA polymerase activity [IDA]
single-stranded DNA binding [IDA]
single-stranded RNA binding [IDA]
translation initiation factor binding [IPI]

Gene Ontology Cellular Component

DNA-directed RNA polymerase II, core complex [IDA]

cytoplasm [IDA]

cytoplasmic mRNA processing body [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Mediator head module structure and functional interactions.

Cai G, Imasaki T, Yamada K, Cardelli F, Takagi Y, Asturias FJ

We used single-particle electron microscopy to characterize the structure and subunit organization of the Mediator Head module that controls Mediator-RNA polymerase II (RNAPII) and Mediator-promoter interactions. The Head module adopts several conformations differing in the position of a movable jaw formed by the Med18-Med20 subcomplex. We also characterized, by structural, biochemical and genetic means, the interactions of the Head module ... [more]

Nat. Struct. Mol. Biol. Mar. 01, 2010; 17(3);273-9 [Pubmed: 20154708]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SRB5 RPB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Liu Y (2001)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SRB5

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]core RNA polymerase II binding transcription factor activity [IDA, IGI]

Gene Ontology Cellular Component

RPB4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA-directed RNA polymerase activity [IDA]single-stranded DNA binding [IDA]single-stranded RNA binding [IDA]translation initiation factor binding [IPI]

Gene Ontology Cellular Component

Synthetic Lethality

Publication

Mediator head module structure and functional interactions.

Throughput

Ontology Terms

Related interactions

Curated By

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
core RNA polymerase II binding transcription factor activity [IDA, IGI]

RNA-directed RNA polymerase activity [IDA]
single-stranded DNA binding [IDA]
single-stranded RNA binding [IDA]
translation initiation factor binding [IPI]