BAIT

SIR2

MAR1, NAD-dependent histone deacetylase SIR2, L000001895, YDL042C
Conserved NAD+ dependent histone deacetylase of the Sirtuin family; involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication; functions as a regulator of autophagy like mammalian homolog SIRT1, and also of mitophagy; SIR2 has a paralog, HST1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

SSA2

YG102, Hsp70 family chaperone SSA2, L000002070, YLL024C
ATP-binding protein; involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

The polarisome is required for segregation and retrograde transport of protein aggregates.

Liu B, Larsson L, Caballero A, Hao X, Oling D, Grantham J, Nystroem T

The paradigm sirtuin, Sir2p, of budding yeast is required for establishing cellular age asymmetry, which includes the retention of damaged and aggregated proteins in mother cells. By establishing the global genetic interaction network of SIR2 we identified the polarisome, the formin Bni1p, and myosin motor protein Myo2p as essential components of the machinery segregating protein aggregates during mitotic cytokinesis. Moreover, ... [more]

Cell Jan. 22, 2010; 140(2);257-67 [Pubmed: 20141839]

Throughput

  • High Throughput

Ontology Terms

  • vegetative growth (APO:0000106)

Curated By

  • BioGRID