BAIT

RMD1

YDL001W

Cytoplasmic protein required for sporulation

GO Process (0)

GO Function (0)

GO Component (1)

Gene Ontology Cellular Component

cytoplasm [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

HAL5

protein kinase HAL5, L000000751, YJL165C

Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication

Kinome Project (Sc)

GO Process (4)

GO Function (1)

GO Component (0)

Gene Ontology Biological Process

cellular cation homeostasis [IGI, IMP]

cellular protein localization [IGI]

protein dephosphorylation [IMP]

protein phosphorylation [IDA]

Gene Ontology Molecular Function

protein kinase activity [IDA, ISS]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

The protein kinase Hal5p is the high-copy suppressor of lithium-sensitive mutations of genes involved in the sporulation and meiosis as well as the ergosterol biosynthesis in Saccharomyces cerevisiae.

Zhao J, Lin W, Ma X, Lu Q, Ma X, Bian G, Jiang L

From a genome-scale genetic screen, we have identified 114 lithium-sensitive and 6 lithium-tolerant gene mutations in Saccharomyces cerevisiae. Twenty-five of these identified lithium-sensitive mutations are of genes previously reported to be involved in sporulation and meiosis, whereas thirty-six of them are of genes involved in the vacuolar protein sorting (VPS) pathway, mainly functioning in the membrane docking and fusion. Accordingly, ... [more]

Genomics May. 01, 2010; 95(5);290-8 [Pubmed: 20206679]

Throughput

Low Throughput

Ontology Terms

phenotype: metal resistance (APO:0000090)

Additional Notes

Overexpression of HAL5 suppresses the lithium sensitivity of the single mutant.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HAL5 RMD1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.1289	BioGRID	389976
RMD1 HAL5	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.1289	BioGRID	365301
HAL5 RMD1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Sharifpoor S (2012)	High	-0.129	BioGRID	912042

Curated By

BioGRID

Interaction Summary