An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability.

Yan Z, Delannoy M, Ling C, Daee D, Osman F, Muniandy PA, Shen X, Oostra AB, Du H, Steltenpool J, Lin T, Schuster B, Decaillet C, Stasiak A, Stasiak AZ, Stone S, Hoatlin ME, Schindler D, Woodcock CL, Joenje H, Sen R, de Winter JP, Li L, Seidman MM, Whitby MC, Myung K, Constantinou A, Wang W

FANCM remodels branched DNA structures and plays essential roles in the cellular response to DNA replication stress. Here, we show that FANCM forms a conserved DNA-remodeling complex with a histone-fold heterodimer, MHF. We find that MHF stimulates DNA binding and replication fork remodeling by FANCM. In the cell, FANCM and MHF are rapidly recruited to forks stalled by DNA interstrand ... [more]

Mol. Cell Mar. 26, 2010; 37(6);865-78 [Pubmed: 20347428]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FANCM RMI1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Ciccia A (2007)	Low	-	BioGRID	-
FANCM RMI1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Deans AJ (2009)	Low	-	BioGRID	-
RMI1 FANCM	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Deans AJ (2009)	Low	-	BioGRID	-
FANCM RMI1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Deans AJ (2009)	Low	-	BioGRID	-
FANCM RMI1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hoadley KA (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FANCM

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

RMI1