An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM.

Ciccia A, Ling C, Coulthard R, Yan Z, Xue Y, Meetei AR, Laghmani el H, Joenje H, McDonald N, de Winter JP, Wang W, West SC

The Fanconi anemia (FA) core complex plays a crucial role in a DNA damage response network with BRCA1 and BRCA2. How this complex interacts with damaged DNA is unknown, as only the FA core protein FANCM (the homolog of an archaeal helicase/nuclease known as HEF) exhibits DNA binding activity. Here, we describe the identification of FAAP24, a protein that targets ... [more]

Mol. Cell Feb. 09, 2007; 25(3);331-43 [Pubmed: 17289582]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FANCM RMI1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Yan Z (2010)	Low	-	BioGRID	-
FANCM RMI1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Deans AJ (2009)	Low	-	BioGRID	-
RMI1 FANCM	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Deans AJ (2009)	Low	-	BioGRID	-
FANCM RMI1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Deans AJ (2009)	Low	-	BioGRID	-
FANCM RMI1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hoadley KA (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FANCM

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

RMI1