BAIT

E2F3

ATE2FA, E2F TRANSCRIPTION FACTOR-3 E2F3, E2F transcription factor 3, F11F19.8, F11F19_8, AT2G36010
E2F transcription factor 3
GO Process (2)
GO Function (3)
GO Component (1)
Arabidopsis thaliana (Columbia)
PREY

DPB

ATDPB, DPB TRANSCRIPTION FACTOR, AT5G03415
transcription factor DP
GO Process (3)
GO Function (2)
GO Component (2)
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Functional modules in the Arabidopsis core cell cycle binary protein-protein interaction network.

Boruc J, Van den Daele H, Hollunder J, Rombauts S, Mylle E, Hilson P, Inze D, De Veylder L, Russinova E

As in other eukaryotes, cell division in plants is highly conserved and regulated by cyclin-dependent kinases (CDKs) that are themselves predominantly regulated at the posttranscriptional level by their association with proteins such as cyclins. Although over the last years the knowledge of the plant cell cycle has considerably increased, little is known on the assembly and regulation of the different ... [more]

Plant Cell Apr. 01, 2010; 22(4);1264-80 [Pubmed: 20407024]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
E2F3 DPB
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
DPB E2F3
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
E2F3 DPB
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
DPB E2F3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
E2F3 DPB
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID