BAIT

PEP4

PHO9, PRA1, yscA, proteinase A, L000001375, YPL154C
Vacuolar aspartyl protease (proteinase A); required for posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; plays a protective role in acetic acid induced apoptosis; synthesized as a zymogen, self-activates
GO Process (4)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

ATG1

APG1, AUT3, CVT10, serine/threonine protein kinase ATG1, L000003955, S000028502, L000004761, YGL180W
Protein serine/threonine kinase; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p; essential for cell cycle progression from G2/M to G1 under nitrogen starvation
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Exit from the golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae.

van der Vaart A, Griffith J, Reggiori F

The delivery of proteins and organelles to the vacuole by autophagy involves membrane rearrangements that result in the formation of large vesicles called autophagosomes. The mechanism underlying autophagosome biogenesis and the origin of the membranes composing these vesicles remains largely unclear. We have investigated the role of the Golgi complex in autophagy and have determined that in yeast, activation of ... [more]

Mol. Biol. Cell Jul. 01, 2010; 21(13);2270-84 [Pubmed: 20444982]

Throughput

  • Low Throughput

Ontology Terms

  • autophagy (APO:0000074)

Additional Notes

  • double mutants show a severe lack of vacuolar autophagic bodies compared to a pep4 single mutant

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PEP4 ATG1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1945BioGRID
2191949

Curated By

  • BioGRID