A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Rad8Rad5/Mms2-Ubc13 ubiquitin ligase complex controls translesion synthesis in fission yeast.

Coulon S, Ramasubramanyan S, Alies C, Philippin G, Lehmann A, Fuchs RP

Many DNA lesions cause pausing of replication forks at lesion sites; thus, generating gaps in the daughter strands that are filled-in by post-replication repair (PRR) pathways. In Saccharomyces cerevisiae, PRR involves translesion synthesis (TLS) mediated by Poleta or Polzeta, or Rad5-dependent gap filling through a poorly characterized error-free mechanism. We have developed an assay to monitor error-free and mutagenic TLS ... [more]

EMBO J. Jun. 16, 2010; 29(12);2048-58 [Pubmed: 20453833]

Throughput

Low Throughput

Ontology Terms

phenotype: chromosome/plasmid maintenance (APO:0000143)

Additional Notes

double mutant shows synthetic defect in the repair of translesion DNA sequences

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ESO1 RAD8	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Chen Z (2012)	High	-	BioGRID	735364

Curated By

BioGRID

Interaction Summary

ESO1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA-directed DNA polymerase activity [ISO]acetyltransferase activity [ISS]peptidyl-lysine N-acetyltransferase activity, acting on acetyl phosphate as donor [IMP]protein binding [IPI]

Gene Ontology Cellular Component

RAD8