An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

TRPA1 is a substrate for de-ubiquitination by the tumor suppressor CYLD.

Stokes A, Wakano C, Koblan-Huberson M, Adra CN, Fleig A, Turner H

Certain TRP cation channels confer the ability to sense environmental stimuli (heat, cold, pressure, osmolarity) across physiological and pathophysiological ranges. TRPA1 is a TRP-related channel that responds to cold temperatures, and pungent compounds that include the cold-mimetic icilin and cannabinoids. The initial report of TRPA1 as a transformation-associated gene product in lung epithelia is at odds with subsequent descriptions of ... [more]

Cell. Signal. Oct. 01, 2006; 18(10);1584-94 [Pubmed: 16500080]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CYLD

Gene Ontology Biological Process

Gene Ontology Molecular Function

Lys63-specific deubiquitinase activity [IDA]proline-rich region binding [IPI]protein binding [IPI]protein kinase binding [IPI]structural constituent of ribosome [IBA]ubiquitin-specific protease activity [IDA]zinc ion binding [IDA]

Gene Ontology Cellular Component

TRPA1