BAIT
RAD53
LSD1, MEC2, SPK1, serine/threonine/tyrosine protein kinase RAD53, L000001573, YPL153C
DNA damage response protein kinase; required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication; activates the downstream kinase Dun1p; differentially senses mtDNA depletion and mitochondrial ROS; required for regulation of copper genes in response to DNA-damaging agents; relocalizes to cytosol in response to hyoxia
GO Process (8)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
EAF3
YPR023C
Component of the Rpd3S histone deacetylase complex; Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
GO Process (8)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- DNA repair [IDA]
- histone acetylation [IDA]
- histone deacetylation [IMP]
- negative regulation of antisense RNA transcription [IMP]
- negative regulation of transcription, DNA-templated [IMP]
- regulation of DNA-dependent DNA replication initiation [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- transcription elongation from RNA polymerase II promoter [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
A DNA integrity network in the yeast Saccharomyces cerevisiae.
A network governing DNA integrity was identified in yeast by a global genetic analysis of synthetic fitness or lethality defect (SFL) interactions. Within this network, 16 functional modules or minipathways were defined based on patterns of global SFL interactions. Modules or genes involved in DNA replication, DNA-replication checkpoint (DRC) signaling, and oxidative stress response were identified as the major guardians ... [more]
Cell Mar. 10, 2006; 124(5);1069-81 [Pubmed: 16487579]
Throughput
- High Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
Additional Notes
- confirmed by RSA and tetrad analysis
Curated By
- BioGRID