BAIT

TT8

BHLH42, F17A8.170, F17A8_170, TRANSPARENT TESTA 8, AT4G09820
transcription factor TT8
Arabidopsis thaliana (Columbia)
PREY

PAP1

ATMYB75, F25P12.92, F25P12_92, MYB DOMAIN PROTEIN 75, MYB75, MYELOBLASTOSIS PROTEIN 75, SIAA1, SUC-INDUCED ANTHOCYANIN ACCUMULATION 1, phosphatidic acid phosphatase 1, production of anthocyanin pigment 1, AT1G56650
transcription factor MYB75
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

MYB75 functions in regulation of secondary cell wall formation in the Arabidopsis inflorescence stem.

Bhargava A, Mansfield SD, Hall HC, Douglas CJ, Ellis BE

Deposition of lignified secondary cell walls in plants involves a major commitment of carbon skeletons in both the form of polysaccharides and phenylpropanoid constituents. This process is spatially and temporally regulated by transcription factors, including a number of MYB family transcription factors. MYB75, also called PAP1 (PRODUCTION OF ANTHOCYANIN PIGMENT1), is a known regulator of the anthocyanin branch of the ... [more]

Unknown Aug. 31, 2010; 0(0); [Pubmed: 20807862]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PAP1 TT8
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-
TT8 PAP1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID