BAIT

DOT1

PCH1, histone methyltransferase DOT1, KMT4, L000004390, L000004273, YDR440W
Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response
Saccharomyces cerevisiae (S288c)
PREY

REV1

L000001615, YOR346W
Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress
GO Process (2)
GO Function (2)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Loss of H3 K79 trimethylation leads to suppression of RTT107-dependent DNA damage sensitivity through the translesion synthesis pathway.

Levesque N, Leung GP, Fok AK, Schmidt TI, Kobor MS

Genomic integrity is maintained by the coordinated interaction of many DNA damage response pathways, including checkpoints, DNA repair processes, and cell cycle restart. In Saccharomyces cerevisiae,, the BRCT domain-containing protein Rtt107/Esc4 is required for restart of DNA replication after successful repair of DNA damage, and for cellular resistance to DNA damaging agents. Rtt107 and its interaction partner Slx4 are phosphorylated ... [more]

Unknown Sep. 01, 2010; 0(0); [Pubmed: 20810656]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: resistance to chemicals (APO:0000087)
  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • deletion of REV1 also blocks the rescue of RTT107 mutants by DOT1
  • double mutants show increased sensitivity to MMS (PubCHEM ID: 4156 CHEBI ID: 25255)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DOT1 REV1
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
239386
REV1 DOT1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
334239

Curated By

  • BioGRID