BAIT

SMA-6

CELE_C32D5.2, C32D5.2
sma-6 encodes a serine/threonine protein kinase that is orthologous to type I TGF-beta receptors; sma-6 activity is required for regulating body length and for proper development of the male tail; sma-6, along with other genes in the TGF-beta Sma/Mab pathway, also regulates reproductive aging; a reduction of TGF-beta pathway genes extends reproductive span by maintaining oocyte and germline quality; sma-6 expression first begins at the 1.5-fold stage of embryogenesis and continues through adulthood; sma-6 is expressed in dorsal and ventral hypodermis, pharyngeal muscle, and the intestine; expression in the hypodermis is necessary and sufficient for body length regulation.
Caenorhabditis elegans
PREY

CFIM-1

CELE_F43G9.5, F43G9.5
F43G9.5 encodes a NUDIX hydrolase that that inhibits DHC-1 in vivo, and is a (perhaps evolutionarily conserved) target of transcriptional activation by DAF-16; F43G9.5(RNAi) suppresses the lethality of conditional dhc-1 mutations, as well as the spindle length and cytokinesis defects of dhc-1(or195), indicating that F43G9.5 negatively regulates dynein; while loss of F43G9.5 function has no obvious effect on lifespan, dauer formation, or fat storage, F43G9.5 is required for embryonic development, fertility, and general health in mass RNAi assays.
Caenorhabditis elegans

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global analysis of genetic interactions in Caenorhabditis elegans.

Byrne AB, Weirauch MT, Wong V, Koeva M, Dixon SJ, Stuart JM, Roy PJ

BACKGROUND: Understanding gene function and genetic relationships is fundamental to our efforts to better understand biological systems. Previous studies systematically describing genetic interactions on a global scale have either focused on core biological processes in protozoans or surveyed catastrophic interactions in metazoans. Here, we describe a reliable high-throughput approach capable of revealing both weak and strong genetic interactions in the ... [more]

J. Biol. Sep. 28, 2007; 6(3);8 [Pubmed: 17897480]

Quantitative Score

  • 2.0 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: organism development variant (WBPHENOTYPE:0000531)

Additional Notes

  • A systematic genetic interaction analysis (SGI) was carried out to detect interactions between 11 query mutants and 858 target genes compromised by RNA interference (RNAi). Interactions were determined using growth scores that indicated whether the resulting number of progeny from the double mutant was significantly different than that of single mutant controls.
  • Negative Genetic

Curated By

  • BioGRID