BAIT
DAF-2
CELE_Y55D5A.5
Protein DAF-2
GO Process (16)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- dauer entry [IMP]
- dauer exit [IMP]
- dauer larval development [IMP]
- determination of adult lifespan [IGI, IMP]
- gamete generation [IMP]
- insulin receptor signaling pathway [ISS]
- lipid storage [IMP]
- locomotion [IMP]
- negative regulation of transcription factor import into nucleus [IGI, IMP]
- positive regulation of multicellular organism growth [IMP]
- protein autophosphorylation [ISS]
- regulation of autophagic vacuole assembly [IMP]
- regulation of development, heterochronic [IGI]
- regulation of response to reactive oxygen species [IMP]
- reproduction [IMP]
- response to heat [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Caenorhabditis elegans
PREY
CFIM-1
CELE_F43G9.5, F43G9.5
F43G9.5 encodes a NUDIX hydrolase that that inhibits DHC-1 in vivo, and is a (perhaps evolutionarily conserved) target of transcriptional activation by DAF-16; F43G9.5(RNAi) suppresses the lethality of conditional dhc-1 mutations, as well as the spindle length and cytokinesis defects of dhc-1(or195), indicating that F43G9.5 negatively regulates dynein; while loss of F43G9.5 function has no obvious effect on lifespan, dauer formation, or fat storage, F43G9.5 is required for embryonic development, fertility, and general health in mass RNAi assays.
GO Process (6)
GO Function (1)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Caenorhabditis elegans
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A global analysis of genetic interactions in Caenorhabditis elegans.
BACKGROUND: Understanding gene function and genetic relationships is fundamental to our efforts to better understand biological systems. Previous studies systematically describing genetic interactions on a global scale have either focused on core biological processes in protozoans or surveyed catastrophic interactions in metazoans. Here, we describe a reliable high-throughput approach capable of revealing both weak and strong genetic interactions in the ... [more]
J. Biol. Sep. 28, 2007; 6(3);8 [Pubmed: 17897480]
Quantitative Score
- 2.6 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: organism development variant (WBPHENOTYPE:0000531)
Additional Notes
- A systematic genetic interaction analysis (SGI) was carried out to detect interactions between 11 query mutants and 858 target genes compromised by RNA interference (RNAi). Interactions were determined using growth scores that indicated whether the resulting number of progeny from the double mutant was significantly different than that of single mutant controls.
- Negative Genetic
Curated By
- BioGRID