An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Regulation of stability of cyclin-dependent kinase CDK11p110 and a caspase-processed form, CDK11p46, by Hsp90.

Mikolajczyk M, Nelson MA

CDK11p110 (cyclin-dependent kinase 11p110, formerly known as PITSLRE) is a member of the CDK superfamily. It associates with cyclin L and is involved in the regulation of transcription and in premRNA splicing. During staurosporine-, Fas- and tumour necrosis factor a-induced apoptosis, CDK11p110, is cleaved by caspases to generate smaller 46-50 kDa proteins containing the catalytic kinase domain. Ectopic expression of ... [more]

Biochem. J. Dec. 15, 2004; 384(0);461-7 [Pubmed: 15344906]

Throughput

Low Throughput

Ontology Terms

a-375 cell (BTO:0002806) [melanoma (DOID:1909)]

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CDK11B HSPA4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Mikolajczyk M (2004)	Low	-	BioGRID	477918

Curated By

BioGRID

Interaction Summary

CDK11B

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase activity [TAS]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

HSPA4