BAIT

ELK1

ELK1, member of ETS oncogene family
GO Process (19)
GO Function (5)
GO Component (2)
Homo sapiens
PREY

HDAC1

GON-10, HD1, RPD3, RPD3L1, RP4-811H24.2
histone deacetylase 1
GO Process (33)
GO Function (18)
GO Component (10)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Temporal recruitment of the mSin3A-histone deacetylase corepressor complex to the ETS domain transcription factor Elk-1.

Yang SH, Vickers E, Brehm A, Kouzarides T, Sharrocks AD

The transcriptional status of eukaryotic genes is determined by a balance between activation and repression mechanisms. The nuclear hormone receptors represent classical examples of transcription factors that can regulate this balance by recruiting corepressor and coactivator complexes in a ligand-dependent manner. Here, we demonstrate that the equilibrium between activation and repression via a single transcription factor, Elk-1, is altered following ... [more]

Mol. Cell. Biol. Apr. 01, 2001; 21(8);2802-14 [Pubmed: 11283259]

Throughput

  • Low Throughput

Curated By

  • BioGRID