BAIT

IRE1

ERN1, bifunctional endoribonuclease/protein kinase IRE1, L000000875, YHR079C

Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress

Kinome Project (Sc)

GO Process (6)

GO Function (5)

GO Component (2)

Gene Ontology Biological Process

endoplasmic reticulum unfolded protein response [IMP]

fungal-type cell wall organization [IGI, IMP]

inositol metabolic process [IMP]

protein homooligomerization [IDA]

protein phosphorylation [IDA]

protein trans-autophosphorylation [IDA]

Gene Ontology Molecular Function

endoribonuclease activity [IDA]
protein homodimerization activity [IDA]
protein kinase activity [IDA]
protein serine/threonine kinase activity [IDA]
unfolded protein binding [IDA, IMP]

Gene Ontology Cellular Component

integral component of endoplasmic reticulum membrane [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

STB6

L000003369, YKL072W

Protein that binds Sin3p in a two-hybrid assay; STB6 has a paralog, STB2, that arose from the whole genome duplication

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Epistatic relationships reveal the functional organization of yeast transcription factors.

Zheng J, Benschop JJ, Shales M, Kemmeren P, Greenblatt J, Cagney G, Holstege F, Li H, Krogan NJ

The regulation of gene expression is, in large part, mediated by interplay between the general transcription factors (GTFs) that function to bring about the expression of many genes and site-specific DNA-binding transcription factors (STFs). Here, quantitative genetic profiling using the epistatic miniarray profile (E-MAP) approach allowed us to measure 48 391 pairwise genetic interactions, both negative (aggravating) and positive (alleviating), ... [more]

Mol. Syst. Biol. Oct. 05, 2010; 6(0);420 [Pubmed: 20959818]

Quantitative Score

-2.556409554 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (epistatic or suppressor interactions) and S score < -2.5 for negative interactions (synthetic sick/lethal interactions).

Curated By

BioGRID

Interaction Summary