AFT1
Gene Ontology Biological Process
- chromosome segregation [IMP]
- establishment of mitotic sister chromatid cohesion [IMP]
- meiotic chromosome segregation [IMP]
- positive regulation of iron ion transport [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation [IMP]
Gene Ontology Molecular Function
CYC8
Gene Ontology Biological Process
- chromatin remodeling [IGI]
- negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter during mitosis [IMP]
- nucleosome positioning [IDA]
- regulation of fatty acid biosynthetic process by regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of response to DNA damage stimulus [IMP]
Gene Ontology Molecular Function- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IDA, IPI]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA, IGI, IPI]
- histone deacetylase binding [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IDA, IPI]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA, IGI, IPI]
- histone deacetylase binding [IDA]
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Epistatic relationships reveal the functional organization of yeast transcription factors.
The regulation of gene expression is, in large part, mediated by interplay between the general transcription factors (GTFs) that function to bring about the expression of many genes and site-specific DNA-binding transcription factors (STFs). Here, quantitative genetic profiling using the epistatic miniarray profile (E-MAP) approach allowed us to measure 48 391 pairwise genetic interactions, both negative (aggravating) and positive (alleviating), ... [more]
Quantitative Score
- -2.84437955 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (epistatic or suppressor interactions) and S score < -2.5 for negative interactions (synthetic sick/lethal interactions).
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
CYC8 AFT1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
CYC8 AFT1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID