ATP6V1A
Gene Ontology Cellular Component
ACTN1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Z disc [IDA]
- cell projection [ISO]
- cortical actin cytoskeleton [ISO]
- cortical cytoskeleton [TAS]
- cytoplasm [ISO]
- dendritic spine [ISO]
- dense core granule membrane [TAS]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- fascia adherens [IDA]
- focal adhesion [ISO, TAS]
- nucleus [ISO]
- secretory granule [NAS]
- stress fiber [IDA, ISO, TAS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Proteomic identification of the TRAF6 regulation of vacuolar ATPase for osteoclast function.
Osteoclasts are cells specialized for bone resorption. For osteoclast activation, tumor necrosis factor receptor-associated factor 6 (TRAF6) plays a pivotal role. To find new molecules that bind TRAF6 and have a function in osteoclast activation, we employed a proteomic approach. TRAF6-binding proteins were purified from osteoclast cell lysates by affinity chromatography and their identity was disclosed by MS. The identified ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID