ATP6V1A
Gene Ontology Cellular Component
ACTB
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- Tat protein binding [ISO]
- kinesin binding [ISO]
- nitric-oxide synthase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- Tat protein binding [ISO]
- kinesin binding [ISO]
- nitric-oxide synthase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
Gene Ontology Cellular Component
- MLL5-L complex [ISO]
- NuA4 histone acetyltransferase complex [ISO]
- axon [ISO]
- blood microparticle [ISO]
- cortical cytoskeleton [IDA]
- cytoplasmic ribonucleoprotein granule [ISO]
- cytosol [IDA]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- membrane [ISO]
- myelin sheath [IDA]
- nuclear chromatin [ISO]
- postsynaptic density [ISO]
- protein complex [ISO]
- ribonucleoprotein complex [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Proteomic identification of the TRAF6 regulation of vacuolar ATPase for osteoclast function.
Osteoclasts are cells specialized for bone resorption. For osteoclast activation, tumor necrosis factor receptor-associated factor 6 (TRAF6) plays a pivotal role. To find new molecules that bind TRAF6 and have a function in osteoclast activation, we employed a proteomic approach. TRAF6-binding proteins were purified from osteoclast cell lysates by affinity chromatography and their identity was disclosed by MS. The identified ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
ATP6V1A ACTB | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.724 | BioGRID | 2661933 |
Curated By
- BioGRID