BAIT

HCK

JTK9, p59Hck, p61Hck, RP5-836N17.3
HCK proto-oncogene, Src family tyrosine kinase
GO Process (30)
GO Function (4)
GO Component (6)
Homo sapiens
PREY

SRC

ASV, SRC1, c-SRC, p60-Src, RP5-823N20.1
SRC proto-oncogene, non-receptor tyrosine kinase
GO Process (50)
GO Function (17)
GO Component (10)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Membrane-anchored Cbl suppresses Hck protein-tyrosine kinase mediated cellular transformation.

Howlett CJ, Robbins SM

The mammalian proto-oncogene Cbl and its cellular homologues in Caenorhabditis elegans (Sli-1) and Drosophila (D-Cbl) are negative regulators of some growth factor receptor signaling pathways. Herein we show that Cbl can negatively regulate another signaling molecule, namely theSrc-family kinase Hck by targeting it for degradation. Hck-mediated cellular transformation of murine fibroblasts is reverted by ectopic expression of a membrane-anchored allele ... [more]

Oncogene Mar. 07, 2002; 21(11);1707-16 [Pubmed: 11896602]

Throughput

  • Low Throughput

Curated By

  • BioGRID