BAIT

SAK1

PAK1, serine/threonine protein kinase SAK1, L000001333, YER129W
Upstream serine/threonine kinase for the SNF1 complex; plays a role in pseudohyphal groth; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication
Kinome Project (Sc)
GO Process (4)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

HAP4

transcription factor HAP4, L000000754, YKL109W
Transcription factor; subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex; involved in diauxic shift
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Shifting the Fermentative/Oxidative Balance in Saccharomyces cerevisiae by Transcriptional Deregulation of the Snf1 Upstream Activating Kinase Sak1p.

Raab AM, Hlavacek V, Bolotina N, Lang C

With the aim to reduce fermentation by-products and to promote respiratory metabolism by shifting the fermentative/oxidative balance we evaluated the constitutive overexpression of the genes SAK1 and HAP4 in Saccharomyces cerevisiae. Sak1p is one of three kinases responsible for phosphorylation, and thereby activation, of the Snf1p complex, while Hap4p is the activator subunit of the transcriptional complex Hap2/3/4/5. We compared ... [more]

Unknown Jan. 21, 2011; 0(0); [Pubmed: 21257817]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: respiratory metabolism (APO:0000102)

Additional Notes

  • overexpression of Hap4 decreases Biomass yield compared to overexpression of Sak1 alone

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SAK1 HAP4
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
518923

Curated By

  • BioGRID